Abstract
Supplementary which could maintain normal physiological mechanisms and functions while aging has drawn our attention due to the population aging in recent years. Probiotics have been believed with desirable properties such as antioxidation and anti-inflammatory for delaying the aging process. However, the age-related experiments conducted in the mammalian models with probiotics were few. In this study, we demonstrated the effects of administration of probiotics Lactobacillus paracasei GKS6 (GKS6) and Bifidobacterium lactis GKK2 (GKK2), respectively, at the dosage of 5.0 × 109 cfu/kg BW/day for fourteen weeks in senescence-accelerated mouse prone 8 (SAMP8) mice. The three-month-old SAMP8 mice were divided into three groups: control, mice fed with GKS6, and mice fed with GKK2. There were ten females and ten males in each group. The SAMP8 mice fed with probiotics GKS6 and GKK2 showed a significantly lower degree of aging followed by Takeda's grading method on the eleventh week of the experiment. The GKK2 group showed significantly increased forelimb grip strength in male SAMP8 mice and muscle fiber number in both genders. Compared to the control, both GKS6 and GKK2 presented a significant increase in liver superoxide dismutase and catalase activities. In addition, a significant decrease in the levels of liver thiobarbituric acid-reactive substances was observed in the probiotics group. These results suggested that probiotics GKS6 and GKK2 could act as antioxidants in delaying the process of aging and preventing age-related muscle loss.
Highlights
Shih-Wei Lin,1 You-Shan Tsai,2 Yen-Lien Chen,2 Ming-Fu Wang,3 Chin-Chu Chen,4,5,6 Wen-Hsin Lin,7 and Tony J
We demonstrated the effects of administration of probiotics Lactobacillus paracasei GKS6 (GKS6) and Bifidobacterium lactis GKK2 (GKK2), respectively, at the dosage of 5.0 × 109 cfu/kg BW/day for fourteen weeks in senescence-accelerated mouse prone 8 (SAMP8) mice. e three-month-old SAMP8 mice were divided into three groups: control, mice fed with GKS6, and mice fed with GKK2. ere were ten females and ten males in each group. e SAMP8 mice fed with probiotics GKS6 and GKK2 showed a significantly lower degree of aging followed by Takeda’s grading method on the eleventh week of the experiment. e GKK2 group showed significantly increased forelimb grip strength in male SAMP8 mice and muscle fiber number in both genders
A total of sixty SAMP8 mice were divided into three groups (n 10 in each gender): control, mice fed with probiotics GKS6, and mice fed with probiotics GKK2. e probiotics were continuously given for fourteen weeks with a dosage of 5.0 × 109 cfu/kg BW/day. e animal protocol in this study has been approved by the Institutional Animal Care and Use Committee (IACUC no. 20170629A02)
Summary
Shih-Wei Lin ,1 You-Shan Tsai ,2 Yen-Lien Chen ,2 Ming-Fu Wang ,3 Chin-Chu Chen ,4,5,6 Wen-Hsin Lin ,7 and Tony J. The reason for age-related muscle decline has not been clear yet, some scientists suggested that oxidative stress, reactive oxygen species (ROS), for example, could affect cell signaling pathways, promote proteolysis, and inhibit protein synthesis in muscle fibers [13, 14]. Another explanation resulted from inflammatory cytokines IL-6 and TNF-α, for example, the introduction of inflammatory markers that could cause muscle breakdown, was being conducted [15, 16]
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