Abstract
The estimation of serum alpha-fetoprotein (AFP) is useful in the diagnosis and monitoring of primary hepatocellular carcinoma, hepatoblastoma, non-seminomatous testicular germ cell tumours and other germ cell tumours. The iCHROMA™ AFP is a lateral flow chromatography, fluorescence immunoassay (FIA) for the quantitative determination of AFP in serum or plasma. In this study, the Boditech iCHROMA™ AFP assay had a very good precision of 9.8%. It showed a very good correlation with the following 12 methods: Abbott Architect (r2 = 0.9705), BioMerieux VIDAS (r2 = 0.9717), Roche Cobas 6000/8000 (r2 = 0.9738), Siemens Centaur XP/XPT/Classic (r2 = 0.9654), Siemens/DPC/Immulite 2000/2500 (r2 = 0.9673), Siemens/DPC/Immulite 1000 (r2 = 0.9670), Beckman Dxl 600/800 (r2 = 0.9676), Roche Elecsys (r2 = 0.9683), Roche Cobas 4000/e411 (r2 = 0.9688), Roche Modular E170 (r2 = 0.9692), SNIBE Maglumi (r2 = 0.9457) and Ortho Vitros 3600/5600/ECi (r2 = 0.9714). In summary, the iCHROMA™ AFP, a rapid point of care test method, has a within-run precision value of less than 10% and excellent correlations with traditional laboratory methods. There is a consistent overestimation with the iCHROMA™ method, which must be taken into consideration when setting a reference range.
Highlights
Alpha-fetoprotein (AFP) is a carcinoembryonic glycoprotein present in abundance in foetal blood, with
Serum AFP estimation is useful in the diagnosis and monitoring of primary hepatocellular carcinoma, hepatoblastoma, non-seminomatous testicular germ cell tumours and other germ cell tumours
We have extensively evaluated the comparative performance of the qualitative point of care device Boditech iCHROMATM for estimating prostate specific antigen (PSA) [8,9,10], vitamin D [11], human chorionic gonadotrophin (HCG) [12], luteinizing hormone (LH) [12], follicle stimulating hormone (FSH) [12], C-reactive protein (CRP) [13], microalbumin [13] and TSH [14], and found a very good correlation with other traditional laboratory methods
Summary
Alpha-fetoprotein (AFP) is a carcinoembryonic glycoprotein present in abundance in foetal blood, with
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