An evaluation of pedicle infiltration organ perfusion, and prevention of vascular collapse during canine kidney procurement for transplantation

Abstract

Three methods of treatment of the donor kidney or its vascular pedicle during the surgical excision and the period of ischemia in renal transplantation have been evaluated. The methods evaluated were: 1. 1. Prevention of renal intravascular collapse. 2. 2. Infiltration of the renal pedicle with procaine plus intraarterial injection of papaverine or acetylcholine. 3. 3. Flush of the kidney with a balanced salt solution at 5°C. Renal blood flow and function of a kidney treated in one of the described methods was compared to its untreated contralateral mate over a two-hour period while both were maintained in identical ex vivo perfusion systems. When the renal ischemia time was 15 minutes no consistent change in the blood flow or renal function was observed in kidneys treated by any of the three described methods. It is concluded therefore that when the period of renal ischemia is kept short these procedures are of no value in preventing immediate posttransplantation renal failure.