An evaluation of level of oxidative stress and protein glycation in nondiabetic undialyzed chronic renal failure patients

Abstract

Background: Chronic renal failure (CRF) patients on prolonged dialysis and with diabetes have been found to have significant alteration in their antioxidant status and protein glycation, but little is known about the same in nondiabetic undialyzed CRF patients. This study was performed to evaluate (a) the levels of oxidative stress and protein glycation in nondiabetic undialyzed CRF patients and (b) the possible influence of oxidative stress on protein glycation. Design and methods: A case control study was performed on 23 nondiabetic undialyzed CRF patients and 17 control subjects. The concentrations of total plasma ascorbic acid, whole blood reduced glutathione (GSH) and plasma lipid peroxides were measured to evaluate the antioxidant status and oxidative stress. The protein glycation was measured from plasma fructosamine normalized for albumin concentrations. A partial correlation analysis was carried out to analyze the effect of oxidative stress parameters on protein glycation. Results: A compromised nonenzymatic defense against free-radical generation was evidenced by decreased concentrations of whole blood GSH and plasma ascorbic acid. The concentrations of lipid peroxides, fructosamine and fructosamine/albumin ratio were significantly ( p<0.05) higher in the CRF group when compared with controls. The partial correlation analysis revealed that oxidative stress influences protein glycation in CRF patients. Conclusions: Increased oxidative stress might have a role in promoting protein glycation in nondiabetic undialyzed CRF patients. The reduction of oxidative stress and protein glycation might open new therapeutic approaches in treating CRF patients.