Abstract
Three commerical latex agglutination kits for rotavirus detection in faeces, Rota Screen (Mercia Diagnostics Ltd), Slidex Rota-kit (bio Mérieux) and Rotalex (Orion Diagnostica) were evaluated and compared with electron microscopy (EM), enzymelinked immunosorbent assay (ELISA) and polyacrylamide gel electrophoresis (PAGE). Of all positives ( N = 102) RotaScreen detected 91%, Slidex 76%, Rotalex 87%, EM 88%, ELISA 99% and PAGE 100%. The detection rate with Slidex was low because many specimens gave non-specific agglutination. Results of the rotavirus negative specimens tested during the study confirmed the high non-specificity rate (14%) with Slidex compared with only 1–2% with RotaScreen and Rotalex. False positive reactions were low (1–3%) with all three latex tests. It was found that nonspecific agglutination and possibly false positive reactions could be removed by fluorocarbon treatment. The results and problems of interpretation of the latex tests are discussed as are user-acceptability and costs.
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