Abstract
Two species of snakes of the genus Trimeresurus, Trimeresurus flavoviridis and Trimeresurus okinavensis, inhabit Amami Oshima Island in the Loo-Choo Islands. These are the common terrestrial venomous snakes on Amami Oshima Island. There is a ring structure on the centra of vertebrae in these snakes. I tried to confirm by in vivo staining that rings on centra were formed annually. Then, age and life-span was estimated by Walford's graphic method. It appears that the age of these snakes can be estimated by adding total ring number +1. The life-span of
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