Abstract
MicroRNAs (miRNAs) regulate gene expression through degradation and/or translational repression of target mRNAs. Dysregulations in the miRNA machinery may be involved in carcinogenesis of colorectal cancer (CRC). The purpose of the current study was to evaluate the DiGeorge syndrome critical region gene 8 (DGCR8) and argonaute 2 (AGO2) mRNA expression in CRC and to evaluate the value of clinical parameters on their expression. We investigated the mRNA expressions of DGCR8 and AGO2 in 60 CRC tissues and adjacent histologically non-neoplastic tissues by using quantitative real-time PCR. Our study revealed that the mRNA expression level of DGCR8 is up-regulated in CRC. However, AGO2 mRNA expression was not significantly altered in CRC tissues. Neither DGCR8 nor AGO2 mRNA expression level was not associated with any clinical parameters, including age, tumor stage, CEA titer, and BMI in CRC cases. However, the mRNA expression levels of DGCR8 and AGO2 were positively correlated to each other. This study demonstrated for the first time that the DGCR8 mRNA expression level was up-regulated in CRC, suggesting its important role in pathobiology of colorectal carcinogenesis.
Highlights
Colorectal cancer (CRC) is a malignant tumor that originates from the epithelium of colon and rectum and the third most common incident cancer among men worldwide [1]
The mRNA expression levels of DiGeorge syndrome critical region gene 8 (DGCR8) and argonaute 2 (AGO2) were quantified by qPCR in paired specimens of human cancerous colorectal tissues and their respective non-neoplastic colorectal tissues from 60 patients with colorectal cancer (CRC)
Our study revealed that DGCR8 mRNA expression was significantly higher in carcinomatous tissues than in the corresponding non
Summary
Colorectal cancer (CRC) is a malignant tumor that originates from the epithelium of colon and rectum and the third most common incident cancer among men worldwide [1]. It has been reported that the five leading primary cancer sites were the stomach, colon and rectum, lung, liver, and prostate in male during 2009 in Korea [2]. The pathogenesis of CRC is intricate and tightly regulated mechanisms, which involve the accumulation of both genetic and epigenetic alterations in the proliferating cells [3]. Gradually accumulating evidences have demonstrated that a wide range of biological processes such as cellular development, differentiation, proliferation, cell death, metabolism, and carcinogenesis are associated with a group of endogenous, small (approximately 17 nucleotides), and noncoding RNAs called microRNAs (miRNAs) [4,5,6]. The biogenesis of miRNA occurs in a well-organized process, referred to as the ‘‘miRNA
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