Abstract
A mitochondria-targetable ratiometric fluorescent probe, HBTPB, has been developed for the detection of H2O2 with a near infrared (NIR) emission and a large Stokes shift (357 nm). Probe HBTPB displays high selectivity toward H2O2 over other reactive oxygen/nitrogen species (ROS/RNS), biological thiols and anions. The detection limit of this probe is as low as 5.9 × 10−7 M. The sensing process confirmed by NMR and mass spectral analysis shows that the addition of H2O2 to the solution of HBTPB triggers an oxidative hydrolysis and a subsequent elimination reaction undergoes to release an ESIPT (excited-state intramolecular proton transfer) featured fluorescent dye. Probe HBTPB is cell permeable and has been applied to sense exogenous and endogenous H2O2 in living A549 cells.
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