Abstract
Extracellular capsular polysaccharide (CPS) produced by Klebsiella pneumoniae capsular type 2 was quantitated by means of an enzyme-linked immunosorbent assay. Microtiter plates were coated with a CPS-specific mouse monoclonal antibody which served to capture CPS present in culture supernatants and biological fluids. A polyclonal rabbit IgG preparation with high antibody content against CPS type 2 was added as a secondary antibody, and bound rabbit IgG was visualized by the addition of alkaline-phosphatase-conjugated anti-rabbit-IgG. Simultaneous testing of standards of purified CPS type 2 permitted the quantitation of CPS in samples of culture fluid, mouse and human plasma, and human urine. Due to its high sensitivity with a lower limit of detectability of 1 ng·ml −1 of CPS, the test system may prove superior to standard chemical methods of CPS quantification.
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