An enzyme-linked immunosorbant assay (ELISA) specific for antibodies to TNP-LPS detects alterations in serum immunoglobulins and isotype switching in C57BL/6 and DBA/2 mice exposed to 2,3,7,8-tetrachlorodibenzo- p-dioxin and related compounds

Abstract

An enzyme-linked immunosorbant assay (ELISA) was developed to detect IgM and IgG antibodies specific for trinitrophenyl-lipopolysaccharide (TNP-LPS). Treatment of C57BL/6 and DBA/2 mice with 2,3,7,8-tetrachlorodibenzo- p-dioxin (TCDD) and other aryl hydrocarbon (Ah) receptor agonists followed by immunization with TNP-LPS resulted in a dose-dependent decrease in serum IgM which paralleled the decrease in the splenic PFC response. The ED 50 values for the IgM and splenic PFCs in C57BL/6 mice for 2,3,7,8-tetrachlorodibenzo- p-dioxin (TCDD), 3,3′,4,4′,5-pentachlorobiphenyl (pentaCB) and 3,3′,4,4′,5,5′-hexaCB were 2.8 and 1.6, 11 and 14, and 25 and 20 μg/kg, respectively; in the less Ah-responsive DBA/2 mice, the ED 50 values were 8.5 and 10, 61 and 69, and 73 and 71 μg/kg, respectively. In addition, treatment of C57BL/6 mice with TCDD resulted in alterations of serum IgG relative to IgM and a delay of isotype switching was observed after immunization and boosting with TNP-LPS. This ELISA may prove to be a useful tool in monitoring immune function during long-term exposure of mice to TCDD and related compounds and exploring the mechanism of Ah receptor-mediated immunosuppression.