An enzyme immunoassay employing monoclonal antibodies and detecting specifically antibodies to classical swine fever virus

Abstract

An enzyme immunoassay (complex-trapping-blocking ELISA, CTB ELISA) for the detection of antibodies against classical swine fever virus (SFV) has been developed. The CTB ELISA employs two monoclonal antibodies directed against different antigenic sites of SFV. A set of 2545 pig sera was tested in the CTB ELISA and in the neutralizing peroxidase-linked assay (NPLA) for neutralizing antibody to SFV. The CTB ELISA and the NPLA confirmed each other in 97% of the sera. The CTB ELISA detects low-level antibodies that can be found early after infection with low-virulent SFV strains or in postvaccination sera or sera with maternal antibodies. The CTB ELISA scored no false-positive results, whereas the NPLA scored 9 sera positive for SFV on a set of 81 pig sera that had antibodies against bovine viral diarrhoea vir