An Enzymatic Preparation of UDP (U-13C) Glucose

Abstract

Uniformly labeled uridine diphosphoglucose (UDP(U-13C)G) was prepared by a two-step enzymatic synthesis. (U-13C) G-6-P was prepared quantitatively by incubating (U-13C) glucose, ATP, MgS04, and hexokinase. UDP(U-13C) Glucose was prepared by incubation of (U-13C)G-6-P with UDPG pyrophosphorylase, phosphoglucomutase, inorganic pyrophosphatase, UTP, and glucose-1, 6-diphosphate in pH 7.5, 100 mM Tris-HCl buffer. After purification over Biogel P-2 and subsequent preparative HPLC, UDP (U-13C)G was obtained in 50% yield. UDP(U-13C)G was characterized by 13C NMR and FAB-MS.