Abstract
Capillary gas chromatography was performed with a Perkin Elmer 8320 instrument in the splitless mode and by using flame ionization detection. The bonded OV-17 fused silica column was 25 m by 0.25 mm i.d. The oven temperature program was: hold 100 ~ for 1.5 min, i00 ~ to 240 ~ at 30~ hold 240 ~ for 5 min. The solvent was vented after a 0.7 min hold. The septa were changed daily and the injection port liner was cleaned and silylanized weekly. This treatment was supplemented by daily injections of I0 ~L of silylating solution. The silylating solution was obtained from Supelco, Inc., Bellefonte, PA, No. 3-3065 . C18 SepPaks were obtained from Waters Assoc., Medford, MA. The ~-glucuronidase (G-0751, partially purified powder from Helix pomatia) and a-naphthyl-~-D-glucuroniC acid sodium salt (N-8378) were obtained from Sigma Chemical Co., St. Louis, MO.
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