Abstract
A method is presented for routine determination of urinary steroids. It is based on the specific enzymatic conversion of the 3α-OH group on the steroid molecule into a 3-keto-group. Quantitation is obtained by measurement of the absorption at 340 nm from NADH, following a simple hydrolysis, extraction and purification. There are, however, disadvantages. Urinary blank values are high and difficult to eradicate without loss of the most polar corticosteroids. For the steroid biochemist the enzymatic method represents an approach that may have a variety of applications.
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