An Enhanced Anti-Tumor Response by Using Bacterial Magnetosomes Gene Administration Platform

Yishu Tang,Dongmei Wang,Shuren Zhang,Chunxia Zhou,Wenbo Ma

doi:10.26502/acbr.5017053

Abstract

DNA vaccination is an effective way to induce specific immunity. In our study, we used magnetosomes (bacterial magnetic particles, BMPs) as the vehicle of a DNA complex of a secondary lymphoid tissue chemokine, human papillomavirus type E7 (HPV-E7) and Ig-Fc fragment (pSLC-E7-Fc) to produce a gene vaccine (BMP-V) for tumor immunotherapy. In a mouse tumour model, intramuscular injection of BMP-V plus magnetic exposure induced E7- specific immunity eliciting to tumor inhibition. Taken together, these results demonstrated that BMP could be applied as a DNA vehicle to induce specific immune effect.

Highlights

DNA vaccines were an effective method to generate antigen-specific immunity
3.1 Intramuscular injection with magnet application resulted in an increased tumour inhibition as compared with other methods of Bacterial magnetic nanoparticles (BMPs)-V administration Mice were challenged with TC-1 tumor cells subcutaneously followed by vaccination with BMP-V, which was administered using various methods
Compared to other delivery methods, we demonstrated that i.m. injection BMP-carrier gene vaccine plus a magnetic field resulted in better inhibited tumour inhibition

Summary

Introduction

DNA vaccines were an effective method to generate antigen-specific immunity. Various factors influence the success of this method, including the electric pulse, buffer, number of pulses, pulse width, cell density and DNA amount. Both systems require specific and expensive instruments [3]. BMPs could been used as effective carriers of drugs and genes to target cancer because of their unique properties, such as paramagnetism, nanoscale size (40–120 nm) and high dispersal quality [4]. We used the DNA vaccine pSLC-E7-Fc to exemplify the BMPs as a gene carrier. Our results illustrated that BMPs represents a novel and simple approach for gene delivery

Materials and Methods

Result

Discussion