Abstract
In this work, we developed a new type of SERS probe, which was composed of glass-SiO2-Au@MBN@Ag nanoparticles (NPs) three-dimensional Surface-enhanced Raman spectroscopy (SERS) substrate. When the laser passed through the quartz glass sheet, on the one hand, the SiO2 NPs supporting the Au@MBN@Ag NPs increase the roughness of the substrate surface, resulting in a large number of hot spots among nanoparticles. On the other hand, based on the focusing effect of silicon dioxide nanospheres, the laser can better focus on the surface of nanoparticles in the inverted SERS probe, thus showing better SERS enhancement. Furthermore, the Au@MBN@Ag NPs core-shell structure was used with 4-mercaptobenzoonitrile (MBN) as an internal standard molecule, and the quantitative determination of tyrosine and urea was realized by internal standard correction method. The standard working curves of the two had good linear correlation with R2 above 0.9555. The detection limits of tyrosine and urea were in the range of 2.85 × 10−10 M~7.54 × 10−6 M, which confirms that this design can be used for quantitative and specific detection of biological molecules, demonstrating great practical significance for the research of diseases such as skin lesions and endocrine disorders.
Highlights
Tyrosine and urea are important biomass in the human body [1]
Many metabolic disorders are associated with abnormal levels of these in body fluids, such as tyrosinemia [2], liver disease [3], neuropsychiatric disorders [4], and end-stage renal disease (ESRD) [5]
Researchers have developed a variety of tyrosine detection methods, such as fluorescence spectroscopy [7], high performance liquid chromatography [8], electrochemical detection [9], colorimetry [10], and chemiluminescence [11]
Summary
Tyrosine and urea are important biomass in the human body [1]. Many metabolic disorders are associated with abnormal levels of these in body fluids, such as tyrosinemia [2], liver disease [3], neuropsychiatric disorders [4], and end-stage renal disease (ESRD) [5]. Researchers have developed a variety of tyrosine detection methods, such as fluorescence spectroscopy [7], high performance liquid chromatography [8], electrochemical detection [9], colorimetry [10], and chemiluminescence [11]. These detection methods often have many disadvantages such as time-consuming detection and complex pretreatment process. On the basis of the above research, we designed a further kind of glass-SiO2 -Au@MBN@Ag. NPs SERS probe in this work. Au@MBN@Ag NPs surface on the back side to detect tyrosine and urea, which formed an endoscope-like SERS probe. Schematic of the synthesis of glass-SiO2 -Au@MBN@Ag SERS probe
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
