Abstract
Chromosomal DNA has been prepared from mycelial spheroplasts of Phytophthora megasperma (isolate 63, chromosome number n = 13–14) (E. M. Hansen, C. M. Brasier, D. S. Shaw, and P. B. Hamm, 1986. Trans Brit. Mycol. Soc. 87, 557–573) and resolved by a pulsed field gel electrophoresis system which uses contour-clamped homogeneous electric fields. Nine chromosomal DNA bands were separated, the smallest being about 1.4 Mb in size: several larger DNAs were unresolved under all conditions tested. The estimated size was based on migration rates relative to those of chromosomal DNA of Saccharomyces cerevisiae, Schizosaccharomyces pombe, and a Neurospora crassa translocation strain which has a minichromosome.
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