An electrophoretic comparison of enzymes in strains of species in the fission yeast genera Schizosaccharomyces, Octosporomyces, and Hasegawaea.

Abstract

A taxonomic study below the generic or at the specific level was made of the electrophoretic patterns of five enzymes in fourteen strains ofSchizosaccharomyces, Octosporomyces, and Hasegawaea species. The five enzymes were glucose-6-phosphate dehydrogenase, 6-phosphogluconatedehydrogenase, hexokinase, phosphoglucomutase, and fumarase. All of the six strains of Schizosaccharomyces pombe examined were linked to each other with a similarity value of 40% or more. The type strain of Schizosaccharomyces malidevorans was closely related to that of S. pombe with a similarity value of 60%. The similarity values of three strains of Octosporomyces octosporus were 80% or more. All of the three strains ofHasegawaea japonica var. japonica examined had a uniform electrophoretic enzyme pattern. The similarity value between the strains of H. japonica var. japonica and H. japonica var. versatilis was 60%. The three species, S. pombe, O. octosporus, and H. japonica had quite different electrophoretic enzyme patterns. Their similarity values were all 0%. The Co-Q systems of the strains were reinvestigated. These data are discussed from the taxonomic point of view.