Abstract
1. 1. Acid phosphatase isozymes from Xenopus laevis tadpole and froglet tissues were studied using polyacrylamide gel electrophoresis. 2. 2. At least six different isozymes could be detected using α-naphthyl acid phosphate as substrate. No single tissue possessed all six, but some tissue specificities were observed as well as the appearance of new isozymes in the same tissue after metamorphosis. Also, substrate specificities could be detected on the gels directly using α- or β-naphthyl acetate, β-glycerophosphate or p-nitrophenylphosphate as substrates. 3. 3. Acid phosphatase activity assayed biochemically was highest in the 20,000 g sedimentable fraction of froglet stomach homogenates and certain of the isozymes were enriched in this fraction. 4. 4. The isozymes resolved from the regressing cement gland had the same electrophoretic mobilities as those found in froglet stomach homogenates, indicating that no unique acid phosphatases detectable with these techniques are utilized during the regression phase of this embryonic structure.
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