Abstract
The surface coat or glycocalyx of animal and plant cells plays an important role in cell properties such as antigenicity, specific permeability, and ATP ase activity. In the spermatozoa the glycocalyx may be of great importance in capacitation and fertilization phenomena. The glycocalyx is rich in fucose, mannose, galactose and sialic acid. These components are diminished during cell division. During spermatogenesis in mammals, spermatocytes represent a long prophase of meiosis. The purpose of this work was to demostrate the possible variations in the amount of glycocalyx in isolated germinal cells during spermatogenesis differentiation.Rat testes from 10, 23, and 38 days old, were finely scissored, in presence of Eagle essential medium, (mechanical treatment) Another similar group of isolated cells was treated with colagenase. (chemical treatment). Once separation of cells was completed, samples were treated for cytochemical electron microscopy using Alcian blue-lanthanum nitrate as previously described.
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Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
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