Abstract
The DNA extruded from partially lysed nuclear plyhedrosis virus (NPV) nucleocapsids was not associated with nucleosome-like particles. This was determined from electron microscope observations using protein monolayer and protein-free preparative techniques and by gel electrophoresis. The nucleocapsids were partially lysed by incubation in distilled water at 4°C. Extrusion of nonsupercoiled DNA from several sites on the nucleocapsid occured and extensively degraded nucleocapsids formed rosette structures. Treatment of purified DNA with poly- l -lysine at 0.1 mg/ml demonstrated that within a positively charged environment, NPV DNA could condense into rods (with a volume less than that of the nucleocapsid) without an absolute requirement for folding proteins.
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