Abstract
Gamma glutamyl transpeptidase (GGT) is a transferase, which is of great importance in sustaining intracellular cysteine and glutathione levels. The abnormal expression of GGT is significantly associated with features of many metabolic syndromes (e.g., hepatocellular carcinoma). Therefore, it is essential to develop methods to detect GGT so as to monitor the physiological or pathological phenomena related to this species. In this work, by making use of a complex formed by Cu2+ and glutathione, which may exhibit excellent voltammetric response, we have proposed a novel potential electrochemical method for the detection of the enzyme. Results show that in the presence of GGT, the formation of Cu2+-glutathione complex on a working electrode will be disrupted, resulting in greatly depressed electrochemical signals. The primary method exhibits some advantages, such as it being fast, cost-efficient, and conveniently operated. It also has the potential to be further developed as an effective method in the quantitative detection of GGT in real samples.
Highlights
Gamma glutamyl transpeptidase (GGT) is a cell membrane-bound enzyme, which is located on the outer surface of cell membranes
GGT plays a key role in the metabolism of GSH, and significantly contributes to sustaining intracellular cysteine and glutathione levels [4]
We propose a novel primary electrochemical method to detect GGT, which may be further developed as an effective method in the quantitative detection of GGT in real samples
Summary
Gamma glutamyl transpeptidase (GGT) is a cell membrane-bound enzyme, which is located on the outer surface of cell membranes. GGT plays a key role in the metabolism of GSH, and significantly contributes to sustaining intracellular cysteine and glutathione levels [4]. It is involved in cancer drug resistance when overexpressed [5,6], and the over expression may provide cancer cells with survival advantages under stress conditions [7]. The diagnosis and treatment methods of HCC have been developing over the last. Electrophoresis and HPLC methods are relatively time consuming and inconvenient, while spectrophotometric and fluorescent measurements need costly apparatus and some toxic chemical reagents (e.g., 5-amino-2-nitrobenzoate), which restrict the development of portable home medical devices. We propose a novel primary electrochemical method to detect GGT, which may be further developed as an effective method in the quantitative detection of GGT in real samples
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