Abstract
Carcinoembryonic antigen (CEA) is an important broad-spectrum tumor marker. The quantitative detection of a low concentration of CEA has important medical significance. In this study, three-dimensional porous graphene-oxide-supported platinum metal nanoparticles (3DPt/HGO) composites were prepared by a wet chemical method and modified on an electrode with enhanced conductivity, a large surface area, and good adsorption of immobilizing antibodies (Ab1). Horseradish peroxidase (HRP)-functionalized Au nanoparticles were fabricated to label the secondary antibodies (Ab2). The proposed immunosensor showed a good linear relationship in the range of 0.001–150 ng/mL for CEA and a detection limit of 0.0006 ng/mL. The immunosensor had high sensitivity, good stability and reproducibility, and has great application prospects for the clinical diagnosis of cancer.
Highlights
Carcinoembryonic antigen (CEA), an important broad-spectrum tumor biomarker, is used as an efficient prognostic indicator in the early clinical diagnosis and disease prevention of pancreatic cancer [1,2], colorectal cancer [3,4], breast cancer [5,6], lung cancer [7,8], and gastric cancer [9,10,11]
New immunosensors fabricated with nanomaterials that have excellent performance for the quick and simple detection of CEA with high sensitivity and selectivity are still urgently needed in the medical field
Three-dimensional porous graphene structures serving as electrode materials are expected to show improved electron transfer and mass transport during the electrochemical detection of tumor markers
Summary
Carcinoembryonic antigen (CEA), an important broad-spectrum tumor biomarker, is used as an efficient prognostic indicator in the early clinical diagnosis and disease prevention of pancreatic cancer [1,2], colorectal cancer [3,4], breast cancer [5,6], lung cancer [7,8], and gastric cancer [9,10,11]. Different strategies have been used to measure CEA, such as radioimmunoassay [12], chemiluminescence analysis [13], enzyme-linked immunosorbent assay [14,15], and electrochemistry [16,17] Among these methods, electrochemical immunosensors [18,19] have attracted considerable attention due to their distinct advantages, such as high selectivity, fast response, low sample requirements, miniaturization, and excellent sensitivity [20,21,22,23]. Three-dimensional porous graphene-oxide-supported platinum metal nanoparticles (3DPt/HGO) composites, with enhanced conductivity and large surface areas, are modified on an electrode in order to immobilize Ab1 and improve the sensitivity of the electrochemical immunosensor
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