Abstract
This paper presents a novel method of fabricating an enzymatic biosensor for breath analysis using chromatography paper as enzyme supporting layer and a liquid phase layer on top of screen printed carbon electrodes. We evaluated the performance with ethanol vapor being one of the breathing ingredients. The experimental results show that our sensor is able to measure the concentration of ethanol vapor within the range of 50 to 500 ppm. These results suggest the ability of detecting breath ethanol, and it can possibly be applied as a generic vapor biosensor to a wide range of diseases.
Highlights
Nowadays, breath analysis for disease diagnosis has attracted attention because it represents a noninvasive diagnostics methodology
We propose a new type of enzyme electrode for detecting substances in the being one of the ingredients of breath, we demonstrate the response, sensitivity, correlation of output gaseous phase based on chromatography paper (ChrPr) enzyme immobilized on screen printed carbon electrodes
A new type of enzyme electrodes, ChrSPCEs which are based on a modified chromatography
Summary
Breath analysis for disease diagnosis has attracted attention because it represents a noninvasive diagnostics methodology. These sensors measure electrical signals such as the current output due to the chemical reactions between the enzymes and target molecules Such enzyme electrodes are fabricated by immobilizing a thin layer of enzyme at the electrode surface. As chromatography paper has high water absorbency properties, wet conditions can simple but effective method to fabricate an enzyme electrode for gaseous detection. We propose a new type of enzyme electrode for detecting substances in the being one of the ingredients of breath, we demonstrate the response, sensitivity, correlation of output gaseous phase based on ChrPr enzyme immobilized on screen printed carbon electrodes. We performed experiments to evaluate the ethanol vapor being one of the ingredients of breath, we demonstrate the response, sensitivity, feasibility of modified ChrPr enzyme electrodes (ChrSPCEs). We performed experiments to evaluate the feasibility of modified ChrPr enzyme electrodes (ChrSPCEs)
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