Abstract
In this work, a sensitive electrochemical DNA sensor based on avidin modified electrode and DNA-functionalized Cds nanoparticle (DFNP) was developed. The DNA-Functionalized Cds nanoparticle contained two kinds of DNA, one was hairpin probe DNA with a biotin at the 3’’ terminal and a thiol at the 5’ terminal, the other is linearity signal DNA. Without hybridized with target DNA, the loop of hairpin impeded biotin linked with avidin on electrode. However, after target hybridization, hairpin was opened and biotin was recognized by avidin resulting in DNA-functionalized Cds nanoparticle was brought on electrode surface. Electrochemical signals of methylene blue (MB) bound to the signal DNA were measured by differential pulse voltammetry (DPV). Introduction By using this new method, we demonstrate that this prototype sensor has been able to detect as low as picomolar p53 tumor suppressor gene with excellent differentiation ability for even single mismatches.
Highlights
The p53 tumor suppressor gene, well known as a transcription factor of cell regulation, is the most commonly mutated gene in human tumors
We reported a non-immobilizing electrochemical DNA sensor with hybridization occurred in one homogeneous solution that employed DNA-functionalized Cds nanoparticle (DFNP) and avidin modified electrode
We have developed a DNA-functionalized Cds nanoparticle, integrated DNA recognition; signal amplification and specific biotin- avdin link functional section, as probe for DNA detection and featured high sensitivity up to low pmtomolar
Summary
The p53 tumor suppressor gene, well known as a transcription factor of cell regulation, is the most commonly mutated gene in human tumors. Sequence-specific analysis of the p53 gene could help early diagnosis of cancer development and increase the success of the treatment sensitive and rapid detection of p53 gene and the mutations in the p53 gene are of great value. We reported a non-immobilizing electrochemical DNA sensor with hybridization occurred in one homogeneous solution that employed DNA-functionalized Cds nanoparticle (DFNP) and avidin modified electrode. We have developed a DNA-functionalized Cds nanoparticle, integrated DNA recognition; signal amplification and specific biotin- avdin link functional section, as probe for DNA detection and featured high sensitivity up to low pmtomolar. Taking advantage of amplification effects of the Cds nanoparticle (CdsNP) and binding specificity of hairpin probe, this biosensor greatly simplifies the electrochemical detection method of DNA and displays higher specificity than the linear probe in DNA detection
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