Abstract
On the basis of gold nanofilm (GNF) electrode and Y junction structure of DNA, a simple and facile single-step DNA sensing protocol with improved sensitivity and lower detection limit was successfully developed. The GNF was pre- pared via rapid electrooxidization of the gold surface followed by the chemical reduction of the produced gold oxide layer. The capture probe DNA (c-DNA) was firstly immobilized onto GNF electrodes via Au—S bonding, for the subsequent forming of Y junction structure with target DNA (t-DNA) and reporter probe DNA (r-DNA) labeled with methylene blue (MB). Harnessing the unique properties of GNF would allow the improved contact of MB with the electrode surface and hence boost the interfacial electron communication. Experimental results of differential pulse voltammetry (DPV) showed that the peak current of the prepared biosensor was linear with the target DNA concentration from 1.0×10 -12 to 1.0×10 -9
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