Abstract
Unreasonable disposal of sulfadoxine (SDX) causing food safety and eventually result water pollution, and shows great significance of detection. A sensitive unit Aptamer (Apt) was adopted to construct an electrochemical sensor BSA/Apt/indole/MWCNTs/GCE with the synergistic amplification of multi-walled carbon nanotubes (MWCNTs) and indole for the detection of SDX. Optimization of the conditions allowed the detection of SDX shows a wide linear range from 0.10 to 1000.00 µmol/L by differential pulse voltammetry (DPV). The LOD and sensitivity were determined to be 0.033 µmol/L and 0.29 µA/(µmol/L·cm²), respectively. The recovery rate of SDX in pork and lake water samples range 98.57 %−104.93 %, 93.6 %−106.2 %, respectively. Both the relative standard deviation (RSD) was lower than 7.40 %, which proved that the sensor has good practicability. In addition, DFT calculation was established that a restricted of B3LYP and a basis set of 6–31 G, the electrooxidation of SDX involves two-electron and one-proton transfer.
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