Abstract
This study develops an electroanalytical sensor using overoxidized polypyrrole-gold composite (Au/PPyox) for the detection of tyramine in fermented foods. Tyramine, a biogenic amine, serves as an essential quality marker, but at higher concentrations can pose health hazards, particularly for individuals with specific metabolic disorders. Current detection methodologies require complex equipment and specialized personnel, impeding their routine use. We synthesized Au/PPyox via oxidative polymerization of pyrrole and developed an Au/PPyox-based sensor, exhibiting substantial selectivity and sensitivity towards tyramine. The sensor exhibited a limit of detection as low as 0.01 μM, demonstrating its high sensitivity. Moreover, it retained stability, selectivity, and reproducibility over two weeks under ambient conditions. Practical application of the sensor in various fermented foods illustrated its potential for routine use. The results indicate that the Au/PPyox-based electroanalytical sensor can serve as a reliable, cost-effective, and user-friendly tool for tyramine detection in fermented foods.
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