Abstract
Miscanthus × giganteus is a perennial grass considered to be one of the most promising biofuel and bioenergy crops because of its high biomass and quality, and low requirement for fertilizers and pesticides. Market demand for Miscanthus is rapidly increasing. However, M. × giganteus is a triploid that cannot produce viable seeds, and it has traditionally been propagated through rhizome division, which is low throughput and labor-intensive. Plant tissue culture provides the potential to propagate M. × giganteus in vitro while maintaining the original plant characteristics. Although protocols exist for M. × giganteus micropropagation, the multiplication rate and plant quality need to be improved to meet commercial demands. For this research, we have assessed callus induction, callus multiplication, plantlet regeneration, shoot multiplication, shoot quality improvement, rooting, plant acclimatization, and survival in the greenhouse and in the field. Through these studies, we have developed an efficient system for high-quality, large-scale micropropagation of M. × giganteus. The plants produced from our protocol exhibited more than 99% survival in soil due to the production of vigorous shoots and roots.
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