An efficient protocol optimization for callus induction, regeneration and acclimatization in sugarcane cv. CO 94012

Abstract

Sugarcane's vast and complex genome, photosensitivity, low seed setting, prolonged crop duration, and complex environmental effect make it difficult to create diverse genetic variability by conventional breeding. However, the in vitro culture system can play a vital role in boosting yield, quality, and making the crop climate smart through in vitro approaches. In the present study in vitro culture using immature leaf sheath of Sugarcane (Saccharum officinarum L. cv- CO 94012) as an ex plant was used to standardize the protocol for callus induction and whole plant regeneration. MS medium supplemented with 2.0 mg/l, 2-4 D, recorded the highest percentage of callus induction. The highest number of multiple shoots was produced on MS medium fortified with KIN 1.0 mg/l. Rooting was more profuse when in vitro regenerated shoots were inoculated on MS basal medium supplemented with 3.0 mg/l NAA. Rooted shoots were hardened in the greenhouse before being transplanted to the field, where 90% survival rate.