An efficient protocol for plant regeneration from cotyledons of Cassia obtusifolia seedlings

Abstract

The purpose of this study was to develop an efficient protocol for plant regeneration from cotyledons of Cassia obtusifolia in order to improve the plant by means of biotechnology. The fragments of cotyledons from aseptic seedlings of C. obtusifolia as explants were cultured in 6 different media, MS basic elements were supplemented with different plant regulators, and calli were developed at a rate of 90–99% of the inoculated explants within 15 days. On MSB regeneration medium, containing MS minerals, B5 organic components, and 0.15 mg L–1 NAA, and 0.5 mg L–1 each of BA, KIN, and ZT, shoots were regenerated from 30% of the calli derived from cotyledon explants. Roots were induced with a frequency of 96% on half-strength MS medium plus 0.2 mg L–1 IBA in 20 days. Complete plants regenerated from cotyledon explants were obtained and transferred to soil pots with high survival ratios.