An Efficient Protocol for Isolation of High Quality Genomic DNA from Seeds of Apple Cultivars (Malus x Domestica) for Random Amplified Polymorphic DNA (RAPD) Analysis

Abstract

Genomic DNA represents the total genome of an organism and is valuable for different molecular studies. The isolation of high quality DNA is a prerequisite for these molecular techniques. The seeds of apple (Malusdomestica) contain high amount of polysaccharides, polyphenols and other secondary metabolites that can hamper DNA isolation, amplification, restriction digestion and subsequent molecular studies. Here, we report a simple, inexpensive and an effi- cient protocol for the isolation of high quality genomic DNA from seeds of different apple cultivars for random amplified polymorphic DNA (RAPD) analysis. This procedure purifies greater amounts of DNA which can be amplified via PCR or digested with endonucleases. The yield of DNA was 200-300 ng/μL (total 8-12 μg DNA from 1-2 apple seeds). The sim- plicity of the procedure makes it very practical for DNA extraction.