An Efficient Protocol for Direct Organogenesis of Pepper ( Capsicum annuum) التکوین العضوى المباشر فى نبات الفلفل

Abstract

A simple and efficient plantlet regeneration protocol was established for Pepper (Capsicum annum L.) achieved through direct organogenesis from Leaves and cotyledons explants. Leaves were taken from 21 day- old in vitro plantlets and and cultured on Murashige and Skoog (1962) MS medium supplemented with different growth regulators. Thidiazuran (TDZ )or Benzyl adenine (BA) with Indole 3-acetic acid (IAA) containing medium was tested. Prolific direct adventitious shoot regeneration occurred on most of the tested growth regulators. The best response in terms of frequency of shoots number per explant (10.5) was obtained from cotyledon cultured on 1.0 mg/L TDZ in combination with 0.5 mg/L IAA. To elongate the shoots it were cultured on MS medium supplemented with various concentrations of gibberellic acid (GA3 ) in combination with 0.25 mg/L Kinetin (KIN). The highest shoot length (2.2 cm) was achieved on MS medium supplemented with 0.5 mg/L GA3 and 0.25 mg/L KIN. Regenerated shoots were excised to root. The highest number of root/shoot was obtained with quarter MS salt medium supplemented with 1.0 mg/L indole-3- butyric acid (IBA) with high length of root (4.60). plantlets were finally transferred to mixture of peatmoss, vermiculite and sand at equal volume 1:1:1.The plantlets showed 80 % survival after 30 days during acclimatization and transplanting.