An Efficient Protocol for Callus Induction in &lt;i&gt;Aquilaria malaccensis&lt;/i&gt; Lam. Using Leaf Explants at Varied Concentrations of Sucrose

Moitreyee Saikia,Karuna Shrivastava,S Sureshkumar Singh

doi:10.5923/j.plant.20120206.03

An Efficient Protocol for Callus Induction in <i>Aquilaria malaccensis</i> Lam. Using Leaf Explants at Varied Concentrations of Sucrose

Moitreyee Saikia, Karuna Shrivastava + Show 1 more

Open Access

https://doi.org/10.5923/j.plant.20120206.03

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Abstract

Aquilaria malaccensis Lam. (Family: Thy melaeaceae), co mmonly known as agarwood, eagle wood or Gaharu is a co mmercially important tree species of northeast India. The Aquilaria tree is categorised as critically endangered in Ind ia. This species is being continuously exploited due to its precious heartwood which is the source of expensive agar oil used in the production of high grade perfu mes as well as in trad itional medicines. The main aim of this study was to establish a speedy callus production protocol fro m Aquilaria malaccensis which may serve as an important option for direct extraction of agar o il. For this purpose, large scale p roduction of callus tissue is needed. An efficient callus regeneration protocol was established through leaf pro liferation in Aquilaria malaccensis Lam. using Murashige and Skoog mediu m supplemented with least amount of plant growth hormones (2,4 D, NAA, Kinetin & BAP) at varied concentrations of sucrose (2-5%). Callus could be initiated in all the treat ments of hormones and sugar levels; however, the best callus growth was obtained in the MS med iu m supplemented with BAP (0.5 mg/l) + NAA (3mg/l) g iving the h ighest fresh (7.368g) and dry cell b io mass (2.170g) at the optimu m sucrose concentration (4%) after 45-60 days of incubation.

Highlights

In Ind ia, th e med iu m s ized ev ergreen t ree Aquilaria malaccensis Lam
Different hormonal co mb inations were tested at their varying concentrations ranging from 0-10mg/l 2,4 -Dichlorophenoxyacetic acid (2,4-D,) + 0-3mg/ l Kinetin (Kn), 0.5-4mg/l 6-benzy ladenine purine (BAP) + 0.5-4mg/l α-naphthalene acetic acid (NAA) and 0-10mg/l 2,4 Dich lorophenoxyacetic acid (2,4-D) + 0.5-4mg/l 6-benzy ladenine purine (BAP) fo r callus induction in A. malaccensis (Table 1)
The surface sterilized explants were placed on MS mediu m[14] supplemented with different concentrations & co mbinations of p lant growth regulators at varying sucrose concentrations (2-5% w/v) to check their effect on callus induction, growth and development[12]

Summary

Introduction

In Ind ia, th e med iu m s ized ev ergreen t ree Aquilaria malaccensis Lam. (syn. Aquilaria agallocha Ro xb. of family Thymelaeaceae) co mmon ly known as agarwood, eagle wood or Gaharu is confined to Eastern Himalayas up to an altitude of 1000m. There are fifteen species in the genus Aquilaria of which eight are known to produce agarwood[2]. The in-vitro propagated medicinal p lants furnish a ready source of uniform, sterile and co mpatible p lant material for mass mu ltiplication and help in germplas m conservation of rare, endangered and threatened medicinal p lants. Taking these factors into consideration, techniques of plant tissue culture were adopted by many scientists for propagation of Aquilaria seedlings and to supplement the conventional methods of regeneration[10],[11]. ; the proper and long-term monitoring of these seedlings is essential fo r their adaptation in natural conditions and for afforestation and plantation programmes fo r the socio-economic benefits of local populace

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