An efficient non-chromatographic method to separate human serum albumin from cryo-poor plasma

Abstract

ABSTRACT Cryo-poor plasma (CPP) which remains after separating cryoprecipitate from the fresh frozen plasma (FFP) unit from human plasma has rare clinical application and can be stored for a maximum of 5 years, depending on storage temperature. In spite of its rare clinical applications that make it as the waste product in blood banking, it has higher concentrations (31.7 g/lit (±0.7)) of albumin than FFP (28.9 g/lit (±0.5)). In this study to develop a rapid, efficient, and inexpensive method to separate human serum albumin (HSA) from CPP, we have benefited from the advantage of the higher thermal stability of HSA. We have investigated the combination of heat treatment of CPP before applying polyethylene glycol as a preferential precipitating agent. In the first step, the effect of various temperatures on proteins’ precipitation profile has been investigated. The resulted effective temperatures (50 and 55 °C) are selected to treat CPP before mixing it with several concentrations and molecular weights of PEG. The pattern of precipitated proteins has been analyzed by the sodium dodecyl sulfate-PAGE and size exclusion chromatography methods. According to achieved data, PEG 8 kDa at concentrations of 20–25% (w/v), with previous heat treatment at 50 °C, showed the significant selective separation of HSA.