An efficient new targeted resequencing method

Abstract

Resequencing of targeted genomic regions, such as exons, enables studies of genetic variation in large numbers of samples. This paper presents a new method called oligonucleotide-selective sequencing (OS-seq) in which oligonucleotides are immobilized on a substrate and used to capture sequences of interest before next-generation sequencing is performed on the same substrate. Additional preparation steps that are involved in other methods are avoided, thus increasing speed. To validate the method, OS-seq was successfully used to target the exons of ~350 cancer genes.