An efficient matrix that resists the nonspecific adsorption of protein to fabricate carbohydrate arrays on silicon

Abstract

Efficient matrix that resists the nonspecific adsorption of proteins from the contacting phosphate buffer silane (PBS) solution has been explored to fabricate carbohydrate arrays on silicon wafer. p-Vinylbenzyllactonoamide (VLA) was immobilized onto silicon through Si–C bond. The VLA-covered substrate was then photolithographically micropatterned through a photomask by ultraviolet (UV) irradiation. The VLA-covered regions recognized specifically the lactose-binding lectin in PBS solution. On the other hand, the UV-irradiated regions, i.e., SiO x surface, prevented the nonspecific adsorption of the lectin from the contacting PBS solution. Several control experiments have demonstrated that the resistant-ability of SiO x surface as the matrix was comparable to the best of known system for resisting the nonspecific adsorption.