An Efficient HPLC–PDA Coupled With Supel™ Tox DON SPE Approach for the Analysis of Deoxynivalenol Contamination in Cereal Grains and Feedstuffs in Jiangxi Province

Abstract

Deoxynivalenol (DON) was commonly found in grains and feedstuffs, which can cause human chronic diseases. In this study, a quick and reliable method was developed for the determination of DON in grains and feedstuffs in Jiangxi Province market. The sample was extracted with acetonitrile–water (84:16, v/v), then purified by Supel™ Tox DON SPE column, and detected by high-performance liquid chromatography (HPLC). The results showed that the calibration curve of DON showed good linearity in the range of 0.01–10.0 μg/mL, and the correlation coefficient R2 = 0.9999. The recovery of DON in the spiked maize sample was 94.8–98.5% by spiking with DON at 0.2 µg/g, 1.0 µg/g, and 2.0 µg/g. The RSD was between 2.5 and 3.3%. This method was used to analyze 120 samples, including 90 grains and 30 feedstuffs, collected from the Jiangxi Province market. The results showed that 81 samples of grains were positive with 90.0% positive rates, and 30 samples of feedstuff were positive with 100% positive rates. Maximum concentration of DON was 0.7 μg/g in oat and 6.9 μg/g in wheat feedstuffs, respectively. Fortunately, the positive samples of grains were safe levels in comparison with National standards for food safety limits of mycotoxins in food (1.0 μg/g), while, the feedstuff of oats was over the Maximum Guideline Level of 16.7% (the Maximum Residue Limit, MRL is 5.0 μg/g). The results of this study about current DON pollution in the grains and feedstuffs on the Jiangxi Province market have not been previously reported.