An efficient enzymatic approach for the detection of glucose and uric acid from blood serum

Abstract

Hydrogen peroxide (H2O2) is one of the significant reactive oxygen species (ROS) in living organisms, which is produced through mitochondrial respiration. The aberrant generation of H2O2 leads to complex diseases. The current study is focused on the preparation of highly effective fluorescent biopolymeric organic nanoparticles for the detection of H2O2. The synthesized compounds are highly selective towards H2O2 in presence of an enzyme horseradish peroxidase (HRP). In-situ generation of fluorogenic polymeric NPs by enzymatic cascade reactions recognize H2O2 in blood serum. This novel approach is suitable for the detection of elevated glucose and uric acid in blood serum in case of diabetic and gout condition through H2O2 detection. This method could hold the place of future diagnostic tool for various complex diseases including diabetes and gout.