An efficient aqueous two phase systems using dual inorganic electrolytes to separate 1,3-propanediol from the fermented broth

Abstract

An aqueous two phase extraction using K2CO3:K2HPO4/Isoproponal was investigated for the recovery of 1,3-propanediol from the fermented broth. Initially, the concentration of K2CO3 on phase formation, the partition co-efficient and recovery of 1,3-PDO was evaluated with a optimum salt concentration of 60%. Later the partition co-efficient was improved using dual inorganic salts, K2CO3 and K2HPO4 with an optimum concentration of 45% and 15% respectively. Using Central Composite Design, pH and temperature on partition and recovery of 1,3-PDO was evaluated. With the optimized physical conditions and inorganic salts concentration, ATPS extraction was carried out in synthetic solution as well as fermented broth resulting in maximum 1,3-PDO partition co-efficient value of 42.46 and 56.93 and recovery yield of 97.69 and 98.27% respectively. A fair partition was observed with organic acids and 1,3-PDO, with removal of lactic acid and acetic acid up to 93.29 and 90.42% respectively.