Abstract
BackgroundFukeqianjin formula (FKQJF) is a Chinese medicine prescription, which has been widely used individually or in combination with other western medicine for the treatment of various gynecological inflammatory diseases, including chronic cervicitis, chronic pelvic inflammatory disease and endometritis, so on and so force.MethodsThe ultra-fast liquid chromatography coupled with triple quadrupole tandem mass spectrometry (UFLC–MS/MS), a quick and efficient method was established and applied to quantify the major constituents of Fukeqianjin formula in rat plasma, and its pharmacokinetics of oral absorption was studied. Nineteen components in Fukeqianjin formula were detected and identified as the major compounds absorbed into the blood according to their chromatographic behavior, molecular weight, ion fragments and other information of these compounds. Furthermore, the plasma drug concentration–time curves were established and the related kinetic parameters were analyzed.ResultsThe results showed that all the 19 compounds could be rapidly absorbed by the gastrointestinal tract, the plasma drug concentration of most compounds could reach a peak at around 1–2 h, and the double-peaks on behalf of the enterohepatic circulation were found in most drug concentration–time curves. The method used in this experiment was validated comprehensively including specificity, linearity, precision, accuracy, stability, matrix effect, and recovery.ConclusionsThese results showed that the developed method was suitable for pharmacokinetic analysis of the main components of Fukeqianjin formula in rat plasma, and may provide useful information for the subsequent distribution studies in vivo.
Highlights
Fukeqianjin formula (FKQJF) is a Chinese medicine prescription, which has been widely used individually or in combination with other western medicine for the treatment of various gynecological inflammatory diseases, including chronic cervicitis, chronic pelvic inflammatory disease and endometritis, so on and so force
It is well known that the multiple constituents contribute to the therapeutic and synergistic effects of traditional Chinese medicine (TCM), and the chemical components absorbed into blood have more possibilities of showing
The diterpenes and flavonoids (7-O-methylwogonin and skullcapflavone I) are considered to be the main bioactive components of Andrographis Herba; the alkaloids and phenol (3,4,5-trimethoxyphenyl-1-Oβ-d-glucopyranoside) originated from Mahoniae Caulis, especially jatrorrhizine, palmatine, and berberine are considered to be the primary bioactive compounds of Mahoniae Caulis.; the phthalide (Z-ligustilide) and phenylpropanoid are the main effective ingredients isolated from Angelicae Sinensis Radix; the flavonoid is the effective ingredient isolated from Moghaniae Radix and the flavonoids originated from Spatholobi Caulis
Summary
Fukeqianjin formula (FKQJF) is a Chinese medicine prescription, which has been widely used individually or in combination with other western medicine for the treatment of various gynecological inflammatory diseases, including chronic cervicitis, chronic pelvic inflammatory disease and endometritis, so on and so force. The diterpenes (neoandrographolide, andrographolide, 14-deoxy-11,12-didehydroandrographolide and andrograpanin) and flavonoids (7-O-methylwogonin and skullcapflavone I) are considered to be the main bioactive components of Andrographis Herba; the alkaloids (methyl-5-hydroxy-2-pyridinecarboxylate, acortatarin A, 9-epi-acortatarin A, jatrorrhizine, palmatine, and berberine) and phenol (3,4,5-trimethoxyphenyl-1-Oβ-d-glucopyranoside) originated from Mahoniae Caulis, especially jatrorrhizine, palmatine, and berberine are considered to be the primary bioactive compounds of Mahoniae Caulis.; the phthalide (Z-ligustilide) and phenylpropanoid (ferulic acid) are the main effective ingredients isolated from Angelicae Sinensis Radix; the flavonoid (genistin) is the effective ingredient isolated from Moghaniae Radix and the flavonoids (ononin and naringenin) originated from Spatholobi Caulis These complex ingredients are the main bioactive components of FKQJF and show a variety of pharmacological effects.
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