Abstract
The quantitative analysis of drug plasma samples plays an important role in the drug development and drug clinical use. Our research team developed a new electrospray ion source―Micro probe electrospray ionization (μPESI) in the early stage, which was combined with mass spectrometry (μPESI-MS/MS) showing good qualitative and quantitative analysis performance. However, matrix effect severely interfered the sensitivity in μPESI-MS/MS analysis. To solve this problem, we recently developed a Solid-phase purification method based on multi-walled carbon nanotubes (MWCNTs), which was used for removing matrix interfering substances (especially phospholipid compounds) in the preparation of plasma samples, so as to reduce the matrix effect.In this study, aripiprazole (APZ), carbamazepine (CBZ) and omeprazole (OME) were used as representative analytes, the quantitative analysis related to the plasma samples spiked with the analytes above and the mechanism of the MWCNTs to reduce matrix effect were both investigated. Compared with the ordinary protein precipitation, MWCNTs could reduced the matrix effect for several to dozens of times, which resulting from the removement of phospholipid compounds from the plasma samples by MWCNTs in the selective adsorption manner. We further validated the linearity, precision and accuracy of this pretreatment technique by the μPESI-MS/MS method. These parameters all met the requirements of FDA guidelines. It was showed that MWCNTs have a good application prospect in the drug quantitative analysis of plasma samples using the μPESI-ESI-MS/MS method.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.