An effective method to generate controllable levels of ROS for the enhancement of HUVEC proliferation using a chlorin e6-immobilized PET film as a photo-functional biomaterial.

Seung Hee Hong,Hakyeong Jeong,Jong-Chul Park,Dohyun Kim,Gyeung Mi Seon,Mi Hee Lee,Min-Ah Koo,Ye Jin Park

doi:10.1093/rb/rbab005

Seung Hee Hong, Hakyeong Jeong + Show 6 more

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https://doi.org/10.1093/rb/rbab005

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Abstract

Reactive oxygen species (ROS) are byproducts of cellular metabolism; they play a significant role as secondary messengers in cell signaling. In cells, high concentrations of ROS induce apoptosis, senescence, and contact inhibition, while low concentrations of ROS result in angiogenesis, proliferation, and cytoskeleton remodeling. Thus, controlling ROS generation is an important factor in cell biology. We designed a chlorin e6 (Ce6)-immobilized polyethylene terephthalate (PET) film (Ce6-PET) to produce extracellular ROS under red-light irradiation. The application of Ce6-PET films can regulate the generation of ROS by altering the intensity of light-emitting diode sources. We confirmed that the Ce6-PET film could effectively promote cell growth under irradiation at 500 μW/cm2 for 30 min in human umbilical vein endothelial cells. We also found that the Ce6-PET film is more efficient in generating ROS than a Ce6-incorporated polyurethane film under the same conditions. Ce6-PET fabrication shows promise for improving the localized delivery of extracellular ROS and regulating ROS formation through the optimization of irradiation intensity.

Highlights

Photodynamic therapy (PDT) is a non-invasive treatment for cancer with minimal side effects
PDT leads to the production of reactive oxygen species (ROS), which play an essential role as regulatory mediators in the signaling process of cells and tissues [6, 7]
A micro BCA assay was conducted to determine the amount of dopamine coated on the polyethylene terephthalate (PET) film [37]

Summary

Introduction

Photodynamic therapy (PDT) is a non-invasive treatment for cancer with minimal side effects. PDT has a relatively low toxic effect on the biological system and does not have a repeatability of cumulative toxicity. PDT leads to the production of reactive oxygen species (ROS), which play an essential role as regulatory mediators in the signaling process of cells and tissues [6, 7]. The effect of PDT is strongly related to the concentration of photosensitizers, which affects the quantity of ROS production. Photosensitizers could only produce a limited amount of ROS at low concentrations, whereas they could generate enough ROS to treat cancer at high concentrations. A high amount of ROS results in apoptosis or necrosis through irreversible damage to biomolecules, such as DNA, protein, and RNA, whereas a low amount of ROS promotes cellular proliferation. Modulating the generation of ROS is vital to their role in cellular function [8,9,10,11,12,13]

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