Abstract
Phospholipids are one of the major bioactive ingredients of Antarctic krill Euphausea superba. A feasible and effective extraction method of Antarctic krill oil was investigated and modified by orthogonal test which the ratio of solid to liquid was 1:2.5, extraction time was 5 min, ratio of ethyl acetate (EA) and n-butanol (BuOH) was 1:1. With this method, the extract of krill oil has a higher phospholipids content of 27.7% - 42.3%, together with total oil yields of 4.15% - 6.18%.
Highlights
Phospholipids are a kind of important bioactive ingredients, and essential nutrients in the metabolism of organisms
In our continuous investigation on feasible and effective extraction method of Antarctic krill oil, an improved solvent extraction and high performance liquid chromatography (HPLC) were used for extraction and detection of the phospholipid from E. superba
Every group was repeatedly extracted in homogenizer with ethyl acetate/n-butanol mixed solvent for 3 times at room temperature
Summary
Phospholipids are a kind of important bioactive ingredients, and essential nutrients in the metabolism of organisms. They were used in food, medicine, and industry widely [1] [2] [3]. In our continuous investigation on feasible and effective extraction method of Antarctic krill oil, an improved solvent extraction and HPLC were used for extraction and detection of the phospholipid from E. superba. Other reagents were analytical grade and obtained from Sinopharm chemical reagent Shanghai co., LTD
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