Abstract
Bisulfite plays crucial roles in diverse physiological processes. Therefore, the efficient detection of bisulfite is very important. In this study, we report a colorimetric and ratiometric fluorescent probe (CPT) with a large Stokes shift (162 nm) for bisulfite (HSO3−) based FRET mechanism. The probe can quantitatively detect HSO3− with low detection limit (45 nM) and high specificity over other common anions and biothiols. A nucleophilic addition reaction was proposed for the sensing mechanism, which was confirmed by HRMS spectra. The test strips of the probe were made and used easily. Moreover, probe CPT was used to ratiometric fluorescent imaging of exogenous and endogenous HSO3− in living cells.
Highlights
Bisulfite (HSO3−), widely used as food preservative because of its antimicrobial, bacteriostasis and antioxidant property[1], is found that its certain concentration level is responsible for respiratory diseases[2,3]
As a continuation of previous work, we report a Förster Resonance Energy Transfer (FRET)-based fluorescent probe composed of coumarin-piperazine-TCF conjugate platform for the colorimetric and ratiometric detection of HSO3−
The absorption maximum of free probe CPT centered at 570 nm and 410 nm, which were assigned to TCF and coumarin moiety, respectively (Fig. 1A)
Summary
Bisulfite (HSO3−), widely used as food preservative because of its antimicrobial, bacteriostasis and antioxidant property[1], is found that its certain concentration level is responsible for respiratory diseases[2,3]. More analytical methods should be developed for trace HSO3−. Many analytical methods including electrochemistry, chromatography, absorption and fluorescence spectroscopy have been developed to detect HSO3− quantitatively[4,5,6,7,8]. One well established method for developing ratiometric probes is based Förster Resonance Energy Transfer (FRET)-an excited-state energy interaction between two fluorophores, wherein the emission profile of one fluorophore (the donor) shows a significant overlap with the excitation profile of the other fluorophore (the acceptor). As a continuation of previous work, we report a FRET-based fluorescent probe composed of coumarin-piperazine-TCF conjugate platform for the colorimetric and ratiometric detection of HSO3−
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