Abstract

Detection of cold allodynia is a very important aspect in the study of pain behavior. An effective and concise device for detecting cold pain has always been the hope of many researchers. Here, an easily produced and operated cold plate device is presented for the assessment of cold allodynia in mice. The device used to detect cold allodynia has two components: a chamber consists of a cylinder for animal experiment and a cube box around the chamber for holding ice to keep temperature stable. In the testing chamber, a mouse was placed on the circular plexiglass plate steady at 4 °C above ice for five minutes. The tested mouse will lift its paw when exposed to the cold plate. The number of lifts will present animal’s response to the degree of cold stimulation. To evaluate this approach, three commonly used pain models of mice were tested: formalin test, bone cancer pain (BCP), and chronic constriction injury (CCI). As is reported in other literatures, these three pain mice models showed increased sensitivity to cold stimulation. The new device is indeed suitable for detecting cold allodynia behavior in mice. Comparisons with existing devices of detecting cold allodynia, such as the cold plate in the market (UGO, Panlab, Columbus, etc.), the new device has the advantages of low cost, simple operation and easy popularization and can detect cold allodynia behavior of mice very well. This is a very practical and economical device to detect cold allodynia behavior.

Highlights

  • Most animal models of acute and chronic pain rely on reflective assays for evaluation of nociception, such as mechanical stimulation, cold stimulation, heat stimulation, and chemical stimulation[1,2]

  • Considering the arbitrary judgment of the acetone test and the cost of the UGO cold plate, we present a new device that can be made and operated: a plexiglass device

  • We present an made device for detection of cold allodynia that objectively reflects cold pain

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Summary

Introduction

Most animal models of acute and chronic pain rely on reflective assays for evaluation of nociception, such as mechanical stimulation, cold stimulation, heat stimulation, and chemical stimulation[1,2] Of all these methods, the detection of cold stimulation is the most difficult to operate because a stable, low temperature is necessary. The acetone test has been adopted by researchers for detecting cold allodynia[3,4,5]. There was another objective method of cold plated testing for detecting cold allodynia. Considering the arbitrary judgment of the acetone test and the cost of the UGO cold plate, we present a new device that can be made and operated: a plexiglass device (temperature of the plate is steady at 4 °C). The new device provides a good way for detection of cold allodynia, and be popularized

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