Abstract
Chlorella vulgaris (Pearsall's strain) grows well on glucose in darkness1. Recently, in this laboratory, we tried to grow this alga on glucose in darkness in an atmosphere free from carbon dioxide. The attempt was unsuccessful; presumably carbon dioxide fixation is essential in at least one of the reactions necessary for growth. Since it has been reported that the requirement for carbon dioxide by Neisseria gonorrhoeae can be replaced by a supplement of yeast extract2, the effect of adding yeast extract to the Chlorella cultures was investigated.
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