An effect of transplantation of human amniotic epithelial cells on Parkinson disease model rats

Abstract

Objective To investigate the survival time of human amniotic epithelial cells(HAECs) after being transplanted into the lateral ventricle of Parkinson disease (PD) model rats,and the effect of HAECs on the circling behavior.MethodsThe Parkinson model was made by using stereotactic microinjection of 6-hydroxydopamine(6-OHDA) into the cerebral striatum.51 PD models were randomly divided into three groups:the HAECs group,the phosphate buffer saline(PBS) group and the normal model group.The HAECs survival expression was investigated in the fifth week by using human specific monoclone antibody nestin and vimentin.After being treated for 10 weeks,the influence of HAECs on the substantia nigra's tyrosine hydroxylase (TH) positive cells of all three groups was collected.Finally,the contents of dopamine (DA)and its metabolite dihydroxyphenylacetic acid(DOPAC) and homovanillic acid(HVA) in the striatum were tested with high performance liquid chromatography-electrochemical detection after being treated for 10 weeks.Results The HAECs can survive for 10 weeks after being transplanted into the lateral ventricle of the PD model rats.The circling behavior of the HAECs group significantly ameliorated than the PBS group and normal model group(P≤0.01).The TH immunohistocbemistry showed that the TH positive cells of HAECs group increased than PBS group and normal model group(P≤0.01).The content of DA,HVA and DOPAC of HAECs group increased than PBS group(P＜0.05～0.01).In addition,the content of DA in the eerebrospinal fluid of HAECs group increased than PBS group(P≤0.01).Conclusions The human amniotic epithelial cells transplantation can improve the cycling behavior of the PD models rats.It may be due to the content of increased DA and its metabolite in the striatum of PD models rats. Key words: Human amniotic epithelial cells; Parkinson disease; Models,animal; Cell transplantation; Dopamine