Abstract
Abstract We investigated an effect of difference in extremities of DNA on its adsorption onto chemically modified cover glass slips. Two kinds of DNA were used. These are λ-DNA and λ-DNA treated with T4 DNA polymerase, which have protruding ends and blunt ends, respectively. The glass substrate surfaces were silanized with 3-aminopropyltrimethoxysilane (APS) or octadecyltrimethoxysilane (OTS). Both DNA molecules were adsorbed on the APS-treated surface due to electrostatic interaction between the positively charged ammonium groups and the negatively charged phosphate groups along the DNA chains, while only untreated λ-DNA was adsorbed on the OTS-treated surface. The latter result is attributable to hydrophobic interaction between the protruding ends and the OTS-treated surface.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
