Abstract
In this paper, we propose and demonstrate the use of an edge-lit volume holographic optical element (EL-VHOE) as a reference waveguide to reduce the volume of a lensless digital holographic microscope. Additionally, a hybrid lensless Fourier transform digital holography is applied to make the EL-VHOE function as an objective turret. It used a spherical wave in the object beam of the EL-VHOE, which served as the reference beam of the microscope. Another sheared spherical wave was used to illuminate the sample. The longitudinal position of the spherical reference beam is changeable. It was shown that the tradeoff between resolution and field of view can be adjusted by changing the longitudinal position of the spherical reference beam. The corresponding experimental results matched the simulational and theoretical predictions. A resolution of approximately 3.11 μm was achieved when the object distance was 6 mm and the longitudinal distance of the spherical reference was 10 mm.
Highlights
In this paper, we propose and demonstrate the use of an edge-lit volume holographic optical element (EL-VHOE) as a reference waveguide to reduce the volume of a lensless digital holographic microscope
We propose and demonstrate the use of an edge-lit volume holographic optical element (VHOE) as a reference waveguide to reduce the volume of an LFT-digital holographic microscope (DHM), and as an objective turret to change the image magnification
An HLFT-DHM is applied to enable the function of an objective turret
Summary
Both the illumination beam of the sample (Objill) and the reference beam of the DHM (R) were divergent spherical waves. The object beam S1VHOE, a spherical wave with its point source apart from the image sensor in the first distance, was incident on the VHP. The interference fringe can be detected only when it is located inside the image sensor, the limit of the sample resolution ( xt ) and the FOV can be calculated as: xt = max 1 − zt ξ , zt zr N ξ. Signal S was simulated as a diffracted beam of the sample illuminated by Objill and propagated to the image sensor plane. It confirms that the FOV in the experiment is approximately the same as that in the simulation result and the theoretical prediction
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